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cDNA Libraries : Methods and Applications
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ISBN: 1617790656 1617790648 9781617790645 Year: 2011 Publisher: Totowa, NJ : Humana Press : Imprint: Humana,

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The numerous vital applications of complementary DNA (cDNA) technology have changed dramatically as the technology has advanced over recent years.  In cDNA Libraries: Methods and Protocols, expert researchers provide current techniques that reflect the latest advances in the construction and application of cDNA libraries.  The first half of the volume covers improved approaches to some of the most basic elements of creating cDNA libraries, while the second half casts a much wider net and includes visionary applications of cDNA technology which were either unforeseen or technically impractical until recently.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, cDNA Libraries: Methods and Protocols serves as an ideal guide to all scientists seeking to advance this important technology and provide answers to the enduring fundamental questions of biology.


Book
Molecular biology of the gene
Authors: --- ---
ISBN: 9780321762436 0321762436 9780321905376 0321905377 9780321902641 0321902645 0321851498 9780321851499 Year: 2014 Publisher: Boston Pearson

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Now completely up-to-date with the latest research advances, the Seventh Edition retains the distinctive character of earlier editions. Twenty-two concise chapters, co-authored by six highly distinguished biologists, provide current, authoritative coverage of an exciting, fast-changing discipline.

Phytochemistry in the Genomics and Post-Genomics Eras
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ISBN: 9780080441160 0080441165 9780080538983 0080538983 9786611056643 1281056642 Year: 2002 Publisher: Burlington : Elsevier Science,

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This monograph series is commissioned by the Phytochemical Society of North America (PSNA). The volumes in this series contain articles on developing topics of interest to scientists, students and individuals interested in recent developments in the biochemistry, chemistry and molecular biology of plants. Volume 36 centers on the role of phytochemistry in the rapid developments in biology brought about by the application of large-scale genomics approaches. Several functional genomic approaches discussed in this volume address plant gene function on a large scale. Plants are combinatori


Book
Viral Vectors for Gene Therapy : Methods and Protocols
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ISBN: 9781617790942 161779094X 9781617790959 1617790958 Year: 2011 Publisher: Totowa, NJ : Humana Press : Imprint: Humana,

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The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection.  In Viral Vectors for Gene Therapy: Methods and Protocols, experts in the field present a collection of their knowledge and experience featuring methodologies that involve virus production, transferring protocols, and evaluating the efficacy of gene products. While thoroughly covering the most popular viral vector systems of adenovirus, retrovirus, and adeno-associated virus, this detailed volume also explores less common viral vector systems such as baculovirus, herpes virus, and measles virus, the growing interest in which is creating a considerable demand for large scale manufacturing and purification procedures.  Written in the highly successful Methods in Molecular Biology™ series format, many chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.   Comprehensive and practical, Viral Vectors for Gene Therapy: Methods and Protocols provides basic principles accessible to scientists from a wide variety of backgrounds for the development of gene therapy viral products that are safe and effective.


Book
Plant Functional Genomics : Methods and Protocols
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ISBN: 1493924443 1493924435 9781493924431 9781493924448 Year: 2015 Publisher: New York, NY : Springer New York : Imprint: Humana,

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This second edition volume discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address specific questions in their favorite model systems. This book is organized into five parts. Part I examines the tools and methods required for identifying epigenetic and conformational changes at the whole-genome level. Part II presents approaches used to determine key aspects of a gene’s function, such as techniques used to identify and characterize gene regulatory networks. This is followed by a discussion of tools used to analyze the levels of mRNA, mRNA translation rates, and metabolites. Part III features a compilation of forward and reverse genetic approaches that include recent implementation of high-throughput sequencing in classical methodologies such as QTL mapping. The final two parts explore strategies to facilitate and accelerate the generation and testing of functional DNA elements, and basic computational tools used to facilitate the use of systems biology approached by a broad spectrum of plant researchers. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and timely, Plant Functional Genomics: Methods and Protocols, Second Edition highlights the latest developments in DNA sequencing technologies that are likely to continue shaping the future of functional genomics.

Keywords

Plant molecular genetics --- Plant gene expression --- Genomics --- Genome --- Eukaryota --- Gene Expression Regulation --- Organisms --- Genetic Processes --- Genetic Structures --- Genetic Phenomena --- Phenomena and Processes --- Gene Expression Regulation, Plant --- Plants --- Genome, Plant --- Botany --- Earth & Environmental Sciences --- Plant Ecology --- Plant molecular genetics. --- Plant gene expression. --- Genomics. --- Génétique moléculaire végétale --- Plantes --- Génomique --- Expression génique --- Plant Genome --- Genomes, Plant --- Plant Genomes --- Plant --- Regulation, Gene Expression, Plant --- Plant Gene Expression Regulation --- Regulation of Gene Expression, Plant --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Expression Regulation, Gene --- Regulation, Gene Action --- Regulation, Gene Expression --- Gene Action Regulation --- Regulation of Gene Expression --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Genomes --- Genome research --- Research --- Life sciences. --- Plant science. --- Botany. --- Life Sciences. --- Plant Sciences. --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Biosciences --- Sciences, Life --- Science --- Floristic botany

Gene Therapy and Gene Delivery Systems
Authors: ---
ISSN: 07246145 ISBN: 9783540284048 3540284044 3540324127 Year: 2005 Volume: 99 Publisher: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer,

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Keywords

Gene Therapy --- Genetic Vectors --- Gene therapy --- Genetic vectors --- Thérapie génique --- Vecteurs de clonage --- Biochemistry --- Biotechnology --- Human genetics --- Medicine --- Microbiology --- Chemistry. --- Human genetics. --- Microbiology. --- Medicine. --- Biotechnology. --- Biochemistry. --- Human Genetics. --- Molecular Medicine. --- Medical Microbiology. --- Biochemistry, general. --- Genetic Structures --- Genetic Phenomena --- Phenomena and Processes --- Biomedical Engineering --- Bioengineering --- Health & Biological Sciences --- Mechanical Engineering --- Engineering & Applied Sciences --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Genetic Processes --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Cloning Vectors --- Shuttle Vectors --- Vectors, Genetic --- Cloning Vector --- Genetic Vector --- Shuttle Vector --- Vector, Cloning --- Vector, Genetic --- Vector, Shuttle --- Vectors, Cloning --- Vectors, Shuttle --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Clinical sciences --- Medical profession --- Microbial biology --- Composition --- Medical microbiology. --- Molecular biology. --- Biology --- Chemistry --- Medical sciences --- Chemical engineering --- Genetic engineering --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Physical sciences --- Molecular biochemistry --- Molecular biophysics --- Biophysics --- Biomolecules --- Systems biology --- Microorganisms --- Life sciences --- Pathology --- Physicians --- Health Workforce


Book
Gene Therapy Protocols : Volume 1: Production and In Vivo Applications of Gene Transfer Vectors
Author:
ISBN: 1597452378 1588299031 9781588299031 9781597452373 9781603272476 Year: 2008 Publisher: Totowa, NJ : Humana Press : Imprint: Humana,

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Abstract

In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today’s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this first volume of Gene Therapy Protocols: Production and In Vivo Applications of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for critical applications in stem cell biology, cancer, diabetes, HIV and tissue engineering. This first volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.

Keywords

Gene therapy --- Gene Transfer Techniques --- Genetic Vectors --- Genetic Techniques --- Genetic Structures --- Genetic Phenomena --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Phenomena and Processes --- Pathology --- Medicine --- Health & Biological Sciences --- Methodology --- Genetic Therapy --- Thérapie génique --- methods. --- Methodology. --- Méthodologie --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Genetic Concepts --- Genetic Phenomenon --- Genetic Process --- Genetic Processes --- Concept, Genetic --- Concepts, Genetic --- Genetic Concept --- Phenomena, Genetic --- Phenomenon, Genetic --- Process, Genetic --- Processes, Genetic --- Genetic Structure --- Structure, Genetic --- Structures, Genetic --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Cloning Vectors --- Shuttle Vectors --- Vectors, Genetic --- Cloning Vector --- Genetic Vector --- Shuttle Vector --- Vector, Cloning --- Vector, Genetic --- Vector, Shuttle --- Vectors, Cloning --- Vectors, Shuttle --- Gene Delivery Systems --- Gene Transfer Technique --- Transgenesis --- Delivery System, Gene --- Delivery Systems, Gene --- Gene Delivery System --- Technique, Gene Transfer --- Techniques, Gene Transfer --- Transfer Technique, Gene --- Transfer Techniques, Gene --- Therapy, Gene --- methods --- Human genetics. --- Animal genetics. --- Gene therapy. --- Human Genetics. --- Animal Genetics and Genomics. --- Gene Therapy. --- Genetic engineering --- Therapeutics --- Heredity, Human --- Human biology --- Physical anthropology

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